If the coding strand in a transcription unit is
5’− ATGCATGCATGCATGCATGCATGCATGC-3’
Then, it is known that the sequence of mRNA is same as the coding strand of DNA.
However, in RNA, thymine is replaced by uracil.
Hence, the sequence of mRNA will be
5’ − AUGCAUGCAUGCAUGCAUGCAUGCAUGC-3’
Hi Kc, No, mRNA is formed complementary to template or non coding strand so there is no requirement for interchange of ends as m RNA sequence is same as of non coding strand except T is replced by U.
TTCAATGATCTG
Shouldn't the 5' and 3' be interchanged?