Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
PCR : Polymerase Chain Reaction.
Multiple copies of the gene of interest is synthesised in vitro using two sets of primers and enzyme DNA polymerase. The enzyme extends the primers using nucleotides provided and genomic DNA as template. The process of DNA replication is repeated several times for amplification of DNA with the help of thermostable DNA polymerase which remains active during high temperature induced denaturation of double stranded DNA.
Fill in the blanks:
(a) Humans reproduce __________. (asexually/sexually)
(b) Humans are__________. (oviparous/viviparous/ovoviviparous)
(c) Fertilization is __________ in humans. (external/internal)
(d) Male and female gametes are __________. (diploid/haploid)
(e) Zygote is __________. (diploid/haploid)
(f) The process of release of the ovum from a mature follicle is called__________.
(g) Ovulation is induced by a hormone called the __________.
(h) The fusion of male and female gametes is called _____________.
(i) Fertilisation takes place in _____________.
(j) Zygote divides to form _____________which is implanted in uterus.
(k) The structure which provides vascular connection between fetus and uterus is called ____________.
Consult internet and find out how to make orally active protein pharmaceutical. What is the major problem to be encountered?