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Class 12th Biology 2016 Set3 Delhi Board Paper Solution

Question 12

Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


PCR : Polymerase Chain Reaction.
Multiple copies of the gene of interest is synthesised in vitro using two sets of primers and enzyme DNA polymerase. The enzyme extends the primers using nucleotides provided and genomic DNA as template. The process of DNA replication is repeated several times for amplification of DNA with the help of thermostable DNA polymerase which remains active during high temperature induced denaturation of double stranded DNA.