Class 12th Biology 2016 Set1 Outside Delhi Board Paper Solution

Question 18

Why is the coding sequence of an enzyme ( β -galactosidase) a preferred selectable marker in comparison to the ones named above ?

Answer

Alternative selectable marker which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this , a recombinant DNA is . inserted within the coding sequence of an enzyme, b galactosidase, which results into activation of the enzyme referred as insertional inactivation coding sequence for the enzyme b-galactosidase is preferred over antibiotic resistance genes because recombinants can be easily visualised.