Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
Below are the steps to be undertaken to obtain a foreign-gene-product:
Step 1. On insertion of a piece of DNA into cloning vector and tranfer into a bacterial plant or animal cell, the alien DNA gets multiplied.
Step 2. After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale.
Step 3. The Cultures may be used for extracting the desired protein and then purifying it by using different separation technique.
Step 4. The cells can also be multiplied in a continous culture system. The used medium is drained out from one side, while fresh medium is added from the other to maintain the cells in their physiologically most active log exponential phase.
Fill in the blanks:
(a) Humans reproduce __________. (asexually/sexually)
(b) Humans are__________. (oviparous/viviparous/ovoviviparous)
(c) Fertilization is __________ in humans. (external/internal)
(d) Male and female gametes are __________. (diploid/haploid)
(e) Zygote is __________. (diploid/haploid)
(f) The process of release of the ovum from a mature follicle is called__________.
(g) Ovulation is induced by a hormone called the __________.
(h) The fusion of male and female gametes is called _____________.
(i) Fertilisation takes place in _____________.
(j) Zygote divides to form _____________which is implanted in uterus.
(k) The structure which provides vascular connection between fetus and uterus is called ____________.
Explain the following terms with example
(a) Co-dominance
(b) Incomplete dominance