While doing a PCR, ‘denaturation&r | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Current NCERT chapter

Academic session 2026-27, Chapter 9 in the current curriculum.

Welcome to the NCERT Solutions for Class 12 Biology - Chapter Biotechnology Principles and Process. This page offers a step-by-step solution to the specific question from Exercise 0, Question 7:

While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

. With detailed answers and explanations for each chapter, students can strengthen their understanding and prepare confidently for exams. Ideal for CBSE and other board students, this resource will simplify your study experience.

Question 7:

While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

Answer:

PCR chain reaction is carried out by the thermostability of the polymerase. PCR contains a series of cycles of three successive steps:- Denaturation, primer annealing and DNA synthesis; These three steps constitutes one complete cycle which is carried out repetitively just by the change in temperature.

Denaturation is the first step of the PCR reaction, in which the strands of duplex DNA are separated by heat at about 93- 95â—¦C. If this separation will not occur then primers will also not be able to anneal to complementary segments on each strand and extension will also not take place. Hence no DNA synthesis will occur and will have negative effect on the process.

Study Tips for Answering NCERT Questions:

NCERT questions are designed to test your understanding of the concepts and theories discussed in the chapter. Here are some tips to help you answer NCERT questions effectively:

  • Read the question carefully and focus on the core concept being asked.
  • Reference examples and data from the chapter when answering questions about Biotechnology Principles and Process.
  • Review previous year question papers to get an idea of how such questions may be framed in exams.
  • Practice answering questions within the time limit to improve your speed and accuracy.
  • Discuss your answers with your teachers or peers to get feedback and improve your understanding.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 7: While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process....

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