Restriction enzymes that are used in the | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Current NCERT chapter

Academic session 2026-27, Chapter 9 in the current curriculum.

Welcome to the NCERT Solutions for Class 12 Biology - Chapter Biotechnology Principles and Process. This page offers a step-by-step solution to the specific question from Exercise 0, Question 4:

Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

. With detailed answers and explanations for each chapter, students can strengthen their understanding and prepare confidently for exams. Ideal for CBSE and other board students, this resource will simplify your study experience.

Question 4:

Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

Answer:

The restriction enzyme cuts double helical segments which contains particular nucleotide sequence which is cut down by the restriction endonuclease enzyme at specific recognition sequence. If the restriction enzyme doesn’t cut the DNA at specific position, it will not produce any sticky ends (cleavage does not falls on symmetrical axis, as in results restriction fragments will give sticky ends) and recombination will also not occur. Instead of giving sticky ends it will produce blunt ends when it cut down from the random position.

cut ends

Study Tips for Answering NCERT Questions:

NCERT questions are designed to test your understanding of the concepts and theories discussed in the chapter. Here are some tips to help you answer NCERT questions effectively:

  • Read the question carefully and focus on the core concept being asked.
  • Reference examples and data from the chapter when answering questions about Biotechnology Principles and Process.
  • Review previous year question papers to get an idea of how such questions may be framed in exams.
  • Practice answering questions within the time limit to improve your speed and accuracy.
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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 4: Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut....

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