A mixture of fragmented DNA was electrop | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Current NCERT chapter

Academic session 2026-27, Chapter 9 in the current curriculum.

Welcome to the NCERT Solutions for Class 12 Biology - Chapter Biotechnology Principles and Process. This page offers a step-by-step solution to the specific question from Exercise 0, Question 8:

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

. With detailed answers and explanations for each chapter, students can strengthen their understanding and prepare confidently for exams. Ideal for CBSE and other board students, this resource will simplify your study experience.

Question 8:

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

Answer:

A mixture of DNA fragments was electrophoresed in agarose gel and after staining with the ethidium bromide, no bands were appeared. There could be many reasons for that and these are following:-

  1. May be the staining concentration is not enough to stain the DNA fragments so that the bands appears.
  2. Isolation process can be degraded and no enzymes worked on break down of cell envelope.
  3. By improper cutting or it could be occur because of endonuclease when cuts on random position or when exonuclease and endonuclease cuts at the same time; or can be loaded in the DNA sample on the agarose gel. 

Study Tips for Answering NCERT Questions:

NCERT questions are designed to test your understanding of the concepts and theories discussed in the chapter. Here are some tips to help you answer NCERT questions effectively:

  • Read the question carefully and focus on the core concept being asked.
  • Reference examples and data from the chapter when answering questions about Biotechnology Principles and Process.
  • Review previous year question papers to get an idea of how such questions may be framed in exams.
  • Practice answering questions within the time limit to improve your speed and accuracy.
  • Discuss your answers with your teachers or peers to get feedback and improve your understanding.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 8: A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethid....

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