Illustrate the design of a bioreactor. H | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

                                               FLASK

                                         BIOREACTOR

It has an agitator system (agitated on shakers) for the mixing of the content properly, an oxygen delivery system to make available of the oxygen, a foam control system, temperature control system, a pH control system and sampling port to withdraw the small volumes of the culture periodically.

Bioreactors are the large vessels that contains 100 to 1000 litres volume which are used for the biological conversion of raw materials into the specific products. It contains cylindrical stirred tank (stirred for mixing) for the mixing of contents as well as the availability of oxygen. And, it contains foam breaker, pH control system and a flat bladed impeller.

These are less advantageous.

These are more advantageous.

Shake flasks are most commonly used small scale culture system for the production.  

Bioreactors are used for the commercial production in which bioprocess is implemented on the plant scale. And it is carried out for the large scale production.

This method is based on the solubility phase technique. In this small volume of culture cannot yield enough quantities of products.

When the foreign DNA inserted into the cloning vector and transferred into the host cell, it produces recombinant DNA and they are allowed to express their role in the desired protein formation.  Then on the small scales they are grown under the controlled condition and finally they extracted and purified from the cultures. The host cell then multiplied in the continuous culture system where the used medium is allowed to pass out form one side and fresh medium is added in culture vessel from another side and this maintains the cell in their most active log/exponential phase. And, larger biomass leads to higher yield of desired proteins.