Class 12 Biology - Chapter Biotechnology Principles and Process NCERT Solutions | A plasmid without a selectable marker wa
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Selectable marker encodes a product which allows the artificial selection and growth of transformed or transfected cells. The cloning vector needs the presence of selectable marker used to identify and eliminate the non- transformant cell. Antibiotic resistance gene are mostly used as selectable markers and as well as antibiotics. A host cell strain is chosen which will react to the particular antibiotics like ampicillin, tetracycline, kanamycin etc. which are been used in transformation process.
So, if there will be no selectable marker, no identification of transformed cell that contains DNA molecule will occur.
Note*:- Ampicillin:- it inhibits the bacterial cell wall synthesis.
Tetracycline:- It inhibits the binding of aminoacyl tRNA to the 30S ribosomal subunit.
Kanamycin:- it inactivates the translation process.
More Questions From Class 12 Biology - Chapter Biotechnology Principles and Process
- Q:- Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
- Q:- What would be the molar concentration of human DNA in a human cell? Consult your teacher.
- Q:- Do eukaryotic cells have restriction endonucleases? Justify your answer.
- Q:- Describe briefly the followings:
(a) Origin of replication
(b) Bioreactors
(c) Downstream processing - Q:- Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
- Q:- Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
- Q:- Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase - Q:- From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
- Q:- Discuss with your teacher and find out how to distinguish between
(a) Plasmid DNA and chromosomal DNA
(b) RNA and DNA
(c) Exonuclease and Endonuclease - Q:- Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
Popular Questions of Class 12 Biology
- Q:- Why is reproduction essential for organisms? Answer
- Q:- Name the parts of an angiosperm flower in which development of male and female gametophyte take place.
- Q:-
Fill in the blanks:
(a) Humans reproduce __________. (asexually/sexually)
(b) Humans are__________. (oviparous/viviparous/ovoviviparous)
(c) Fertilization is __________ in humans. (external/internal)
(d) Male and female gametes are __________. (diploid/haploid)
(e) Zygote is __________. (diploid/haploid)
(f) The process of release of the ovum from a mature follicle is called__________.
(g) Ovulation is induced by a hormone called the __________.
(h) The fusion of male and female gametes is called _____________.
(i) Fertilisation takes place in _____________.
(j) Zygote divides to form _____________which is implanted in uterus.
(k) The structure which provides vascular connection between fetus and uterus is called ____________. - Q:- What do you think is the significance of reproductive health in a society?
- Q:- Mention the advantages of selecting pea plant for experiment by Mendel.
- Q:- Group the following as nitrogenous bases and nucleosides:
Adenine, Cytidine, Thymine, Guanosine, Uracil and Cytosine. - Q:- Explain antibiotic resistance observed in bacteria in light of Darwinian selection theory.
- Q:- What are the various public health measures, which you would suggest as safeguard against infectious diseases?
- Q:- Explain in brief the role of animal husbandry in human welfare.
- Q:- Bacteria cannot be seen with the naked eyes, but these can be seen with the help of a microscope. If you have to carry a sample from your home to your biology laboratory to demonstrate the presence of microbes under a microscope, which sample would you carry and why?
Recently Viewed Questions of Class 12 Biology
- Q:- Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli ?
- Q:- Briefly describe various steps involved in plant breeding.
- Q:- What are sacred groves? What is their role in conservation?
- Q:-
Define external fertilization. Mention its disadvantages.
- Q:- Describe the post-fertilization changes in a flower.
- Q:- Find out from newspapers and popular science articles any new fossil discoveries or controversies about evolution.
- Q:- Differentiate between microsporogenesis and megasporogenesis. Which type of cell division occurs during these events? Name the structures formed at the end of these two events.
- Q:- List all the wastes that you generate, at home, school or during your Trips to other places, could you very easily reduce? Which would be Difficult or rather impossible to reduce?
- Q:- Discuss the role of women and communities in protection and conservation of forests.
- Q:- Differentiate between a zoospore and a zygote.
- All Chapters Of Class 12 Biology
- All Subjects Of Class 12